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. 2020 Jul 18;29(R1):R89–R99. doi: 10.1093/hmg/ddaa154

Figure 2.

Figure 2

Schematic representation of MECP2 3′UTR. Light blue rectangle: the longest MECP2 3′UTR isoform. Above are the approximate localizations of miRNA seed-sequences predicted for MECP2 3′UTR based on TargetScan using high stringency parameters (101). In blue and pink, miRNAs and RBPs demonstrated to regulate MECP2 mRNA stability and/or translation efficiency, respectively. Below are approximate locations of mutations detected in RTT patients without alterations in the MECP2 coding sequence. In red, mutations associated with decreased MECP2 mRNA levels in patients (81). Green and orange arrowheads represent pA sites annotated by Coy et al. (72) and Wang et al. (73), respectively, the latter using 3′-READS assays from multiple cell types. Bottom graph: conservation degree of human MECP2 3′UTR compared to other 100 different vertebrate species shows areas of high similarities. Interestingly, most of these highly conserved sequences overlap with pA sites. The conservation score was calculated using the UCSC phastCons conservation score tool averaged in 100-nt bins (102).