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. 2020 Jul 28;29(16):2723–2735. doi: 10.1093/hmg/ddaa163

Figure 6.

Figure 6

Adult foxc1a+/−; foxc1b−/− fish display myocardial defects. (A and B) MF20 staining of heart histological sections of 10-mpf foxc1a+/+;foxc1b+/− sibling (control) and foxc1a+/−;foxc1b−/− fish. (C and D) DAPI staining of heart histological sections of 10-mpf control and foxc1a+/−;foxc1b−/− fish. (E and F) Quantification (in pixels) of compact zone (MCZ) thickness and trabecular zone (MTZ) thickness in 10-mpf adult fish (n = 7). foxc1a+/−;foxc1b−/− fish have a significantly larger compact zone thickness (E) but unchanged trabecular zone thickness (F); *P = 0.047. (G–J) Quantification (in pixels) of OFT valve area, OFT valve cells/area, AV valve area and AV valve cells/area in 10-mpf control and foxc1a+/−;foxc1b−/− adult fish (n = 3–5). foxc1a+/−;foxc1b−/− fish did not show any statistically significant differences from controls; however, the OFT valve area was larger and this difference approached significance (P = 0.0539) (G). MCZ: myocardial compact zone. MTZ: myocardial trabecular zone. OFT: outflow tract. AV: atrioventricular.