Fig. 3.

Spatial distribution of AQPs that function as H₂O₂ channels.

Immunostaining of AQPs was performed to investigate the contribution of AQPs to H₂O₂ permeability. A: Immunostaining of AQP3 in HeLa and SAS ρ⁰ cells. B: Relative fluorescence intensity of AQP3 in HeLa and SAS ρ⁰ cells. C: Immunostaining of AQP5. D: Relative intensity of AQP5. E: Immunostaining of AQP8. F: Relative fluorescence intensity of AQP8. In HeLa and SAS ρ⁰ cells, AQPs were strongly expressed in the plasma membrane, and average expression intensities were significantly higher than in parental cells. **: p < 0.01 using Student’s t-test (vs. parent).