Fig. 4.

AQP3, 5, and 8 directly bind to NOX2, which produces H₂O₂ in the cell.

Western blot analysis of AQPs was performed to investigate protein expression, and immunoprecipitation was performed to confirm if AQP and NOX2 directly interact. A: Western blot and immunoprecipitation of AQPs and NOX2. AQP3, 5, and 8 directly bound with NOX2. To investigate the spatial distribution of NOX2, immunostaining was also performed. B: Immunostaining of NOX2 in HeLa and SAS ρ⁰ cells. C: Relative fluorescence intensity of NOX2 in HeLa and SAS ρ⁰ cells. NOX2 expression was significantly higher than in parental cells. **: p < 0.01 using Student’s t-test (vs. parent).