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. 2020 Oct 1;10:16261. doi: 10.1038/s41598-020-72328-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SCMA HCV recoveries for different process conditions. Recovery was calculated by relating amounts of genotype 1a HCV RNA in flow through, wash and elutions at 0.6, 1.2, and 2.0 M NaCl to the total RNA amount prior to SCMA. All preceding SXC runs were performed at pH 9 (eluate fractions of Fig. 3), without nuclease treatment prior to SXC (left and middle bar), including intermediate freezing (left bar) and with nuclease treatment preceding SXC (right bar). Bar captions state the order, in which steps were performed. Error values are means of technical triplicates with error bars reflecting SD.