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. 2020 Oct 1;10:16217. doi: 10.1038/s41598-020-73026-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Study design and timeline of the in vivo experiment; (B) Scanning electron microscopy images of the empty and sclerostin antibody (Scl-Ab) PLGA microspheres (MSs) at low and high magnifications. Scale bars indicate 100 μm (left) and 20 μm (right); (C) Mean size (± SD) of empty MS and Scl-Ab MS (empty MS: n = 3; Scl-Ab MS: n = 4); (D) 18-week time course of Scl-Ab release from PLGA MS at 37 °C. * = p < 0. 05.