Table 1.

Numbers of uORFs, protein-coding genes, and assembled EST/TSA and RefSeq sequences extracted at each step of ESUCA.

Step	Drosophila melanogaster			Danio rerio			Gallus gallus			Homo sapiens
	uORFa	Gene	EST/TSA + RefSeq	uORFa	Gene	EST/TSA + RefSeq	uORFa	Gene	EST/TSA + RefSeq	uORFa	Gene	EST/TSA + RefSeq
Before selection	–	13,938	–	–	25,206	–	–	14,697	–	–	19,956	–
Step 1	17,035	7066	–	39,616	14,453	–	8929	3535	–	44,085	12,321	–
Step 2	5040	2343	–	3599	2323	–	1320	767	–	15,069	6568	–
Step 3.1	4900	2308	1,854,900	3494	2271	1,822,408	1275	751	668,417	14,529	6408	7,577,191
Step 3.2	4882	2297	873,484	3479	2261	846,829	1271	750	314,665	14,499	6399	3,711,515
Step 4.1	4307	2076	40,982	2549	1689	37,125	1122	668	42,622	13,993	6217	383,797
Step 4.2	4294	2067	40,894	2543	1688	36,434	1119	665	41,306	13,970	6215	378,480
Step 4.3	49	40	1212	408	343	4082	774	485	8171	5262	3067	33,776
Step 5	49	40	1212	192	180	2798	261	221	4074	1495	1201	12,402
Step 7	37	36	1072	156	154	2729	230	209	3945	1094	969	9964

^aWhen multiple uORFs in a transcript shared the same stop or start codon, they were counted as one.