Fig. 2.

Synergistic interaction between EPI‐7170 and ENZ in ENZR cells. (A) VCaP‐ENZR and (B) C4‐2B‐ENZR cells were treated with DMSO, ENZ, EPI‐7170, or its combination at the indicated concentrations for 5 days (for VCaP‐ENZR) or 2 days (for C4‐2B‐ENZR), and cell proliferation was analyzed by BrdU incorporation assay. IC50s of ENZ alone or in the presence of EPI‐7170 are shown in the tables. (C) Combination index (CI) versus fraction‐affected (Fa) plots for VCaP‐ENZR and (D) C4‐2B‐ENZR were generated by CompuSyn software. CI < 1 represents synergism, CI = 1 additive effect and CI > 1 antagonism. Values represent the mean ± SEM (n = 3).