Fig. 3.

Blocking the transcriptional activities of FL‐AR and AR‐V7. (A) Levels of AR‐V7 ectopically expressed in LNCaP cells were comparable to levels of endogenous AR‐FL. A representative western blot from n = 3 independent experiments is shown. (B) PSA(6.1 kb)‐luciferase and (C) V7BS₃‐luciferase activities in cells treated with DMSO, ENZ (5 µm), EPI‐7170 (5 µm), or the combination in the presence or absence of 1 nm R1881 for 24 h. 1‐fold was set for samples treated with (+) R1881, (−) ENZ, (−) EPI. Statistical significance: * indicates vs DMSO control. # indicates vs ENZ treatment group. n.s., not statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001; ^# P < 0.05; ^### P < 0.001. ANOVA test with Tukey's test. Values represent the mean ± SEM (n = 3).