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. 2020 Aug 9;14(10):2455–2470. doi: 10.1002/1878-0261.12770

Fig. 4.

Fig. 4

Effect of combined ENZ/EPI‐7170 treatments on expression of FL‐AR and AR‐V7 target genes. (A) Levels of FL‐AR and AR‐V7 proteins in VCaP‐ENZR and (B) C4‐2B‐ENZR cells that were treated with DMSO, ENZ, EPI‐7170, or its combination in the presence or absence of 1 nm R1881 for 2 days. A representative western blot from n = 3 independent experiments is shown. (C) mRNA levels of genes regulated by FL‐AR or (D) AR‐V7 in VCaP‐ENZR cells. (E) mRNA levels of genes regulated by FL‐AR or (F) AR‐V7 in C4‐2B‐ENZR cells. Levels of mRNA were normalized to RPL13a with 1‐fold set for samples treated with (+) R1881, (0 µm) ENZ, (0 µm) EPI. Statistical significance: * indicates vs DMSO control. # indicates vs ENZ treatment group. † indicates vs EPI‐7170 treatment group. *P < 0.05; **P < 0.01; ***P < 0.001; # P < 0.05; ## P < 0.01; ### P < 0.001; † P < 0.05; †† P < 0.01; ††† P < 0.001. ANOVA test with Tukey's test. Bars represent the mean ± SEM (n = 3).