Fig. 3.

PGM5‐AS1 upregulation exacerbated osteosarcoma cell EMT, invasion, and migration. oe‐NC or oe‐PGM5‐AS1 was delivered into U2OS and HOS cells. (A) Expression of PGM5‐AS1 in U2OS and HOS cells, as measured using RT‐qPCR. (B) Relative mRNA expression of EMT‐related genes in U2OS and HOS cells detected by RT‐qPCR. (C) Protein expression of EMT‐related genes in U2OS and HOS cells detected by western blot analysis. (D) Migration ability of U2OS and HOS cells detected by Transwell assay (200×; scale bar = 50 μm). (E) Invasion ability of U2OS and HOS cells detected by Transwell assay (200×; scale bar = 50 μm). *P < 0.05, vs. U2OS and HOS cells treated with sh‐NC. The measurement data were presented as mean ± standard deviation. One‐way ANOVA along with Tukey's post hoc test was conducted to analyze the data among multiple groups. The experiment was repeated three times.