Fig. 8.

Tumorigenesis of osteosarcoma cells in vivo is suppressed by the downregulation of PGM5‐AS1 or FBN1 or overexpression of miR‐140‐5p. (A) Tumor volume and tumor weight of the nude mice. (B) Expression of PGM5‐AS1, FBN1, and miR‐140‐5p in developed tumors detected by RT‐qPCR. (C) Expression patters of genes related to EMT including E‐cadherin, N‐cadherin, and Vimentin tested using RT‐qPCR. (D) Protein expression of EMT‐related genes (E‐cadherin, N‐cadherin, and Vimentin) as measured by western blot analysis. (E) Micrographs (left) and number (right) of tumor lung metastases of nude mice (400×; scale bar = 25 μm). *P < 0.05, vs. the nude mice injected with sh‐NC‐treated U2OS cells. The data were measurement data and expressed as mean ± standard deviation. Unpaired t‐test was performed to compare the data between two groups, and repeated measurement ANOVA along with Bonferroni's post hoc test was conducted for analysis of data at different time points. n = 12.