Figure 4. Disruption of p53 and ATR Restores IR-Induced Inflammatory Signaling in c-NHEJ-Deficient MCF10A Cells.
(A–C) WT cells, p53 KO cells, XRCC4 KO cells, or XRCC4 P53 DKO (double knockout) cells were irradiated with 10 Gy (IR), and then maintained in medium with or without ATR inhibitor for 3 days before fixation for immunofluorescence staining. Mean values and SEM are plotted (n = 3).
(D and E) WT cells, p53 KO cells, XRCC4 KO cells or XRCC4 P53 DKO cells were irradiated with 10 Gy and then cultured for 3 days in the presence or absence of ATR inhibitor before collection for western blot analysis.
(F) Bar plot showing the normalized enrichment score (NES) and false discovery rate (FDRq) for the identified enriched biological pathways in MCF10A cells 3 days after 10 Gy irradiation.