KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| RIG-I (D33H1G) | CST | Cat# 42GGS; RRID:AB_21757G6 |
| GAPDH (14C1G) | CST | Cat# 2118S; RRID:AB_1G693448 |
| pSTAT1 Y7G1 (58D6) | CST | Cat# 9167S; RRID:AB_1G86GG71 |
| cGAS (D1D3G) | CST | Cat# 151G2S; RRID:AB_2799712 |
| STING (D2P2F) | CST | Cat# 13647; RRID:AB_2799947 |
| IFITM1 | Proteintech | Cat# 11727–3-AP; RRID:AB_2122G83 |
| ISG54 | Proteintech | Cat# 126G4–1-AP |
| ISG56 | Thermo | Cat# PA3–848; RRID:AB_1958733 |
| XRCC4 | BD | Cat# 6115G6; RRID:AB_398966 |
| CENP-F | BD | Cat# 61G768; RRID:AB_398G91 |
| gH2AX (JBW3G1) | Millipore | Cat# G5–636; RRID:AB_3G9864 |
| RIG-I (1C3) | Millipore | Cat# MABF297; RRID:AB_265G546 |
| p53 (DO-1) | Santa Cruz | Cat# sc-126; RRID:AB_628G82 |
| p21 (F-5) | Santa Cruz | Cat# sc-6246; RRID:AB_628G73 |
| Pol III RPC32 (H-9) | Santa Cruz | Cat# sc-48365; RRID:AB_2165718 |
| MAVS (E-3) | Santa Cruz | Cat# sc-166583; RRID:AB_2G123GG |
| Ku7G | Bethyl | Cat# A3G2–624A; RRID:AB_1G554672 |
| CTLA4 (CD152) | BioXCell | Cat# BEG131; RRID:AB_1G95G184 |
| pH3Ser1G (D2C8) | CST | Cat# 3465S; RRID:AB_1G69586G |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2'3'-cGAMP | InvivoGen | Cat# tlrl-nacga23 |
| 5'ppp-dsRNA | InvivoGen | Cat# tlrl-3prna |
| RO-33G6 | Selleck Chemical | Cat# S7747 |
| SiR-DNA | Cyotskeleton | Cat# CY-SCGG |
| Ku55933 | Selleck Chemical | Cat# S1G92 |
| VE-821 | Selleck Chemical | Cat# S8GG7 |
| CHIR-124 | Selleck Chemical | Cat# S2683 |
| LY26G3618 | Selleck Chemical | Cat# S2626 |
| BI2536 | Selleck Chemical | Cat# S11G9 |
| ML-6G218 | Millipore | Cat# 5574G3 |
| Shield-1 | Aobious | Cat# AOB1848 |
| 4-OHT | Sigma | Cat# H79G4 |
| Critical Commercial Assays | ||
| TruSeq® Stranded mRNA Library Prep | Illumina | Cat# 2GG2G594 |
| NextSeq 500/550 High Output Kit v2.5 (75 Cycles) | Illumina | Cat# 2GG249G6 |
| Mitochondria isolation kit | Thermo | Cat# 89874 |
| miRNeasy Mini Kit | QIAGEN | Cat# 217GG4 |
| Deposited Data | ||
| RNA-seq data | This paper | GEO: GSE145148 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6 mice, female | Charles River | Strain code: G27 |
| Oligonucleotides | ||
| p53#1 F | IDT DNA | CACCGATCCACTCACAGTTTCCAT |
| p53#1 R | IDT DNA | aaacATGGAAACTGTGAGTGGATC |
| p53#2F | IDT DNA | CACCGAGCACATGACGGAGGTTGTG |
| p53#2 R | IDT DNA | aaacCACAACCTCCGTCATGTGCTC |
| RIG-I#A F | IDT DNA | CACCGCTAGGGCATCCAAAAAGCCA |
| RIG-I#A R | IDT DNA | aaaclGGCIIIIIGGAIGCCCI AGC |
| RIG-I#B F | IDT DNA | CACCGAGATCAGAAATGATATCGGT |
| RIG-I#B R | IDT DNA | aaacACCGATATCATTTCTGATCTC |
| Ku70 F | IDT DNA | CACCGGAGTCCTACTACAAAACTG |
| Ku70 R | IDT DNA | aaacCAGTTTT GTAGTAGGACTCC |
| Recombinant DNA | ||
| LentiGuide Puro | Addgene | Cat# 52963 |
| LentiCas9 Blast | Addgene | Cat# 52962 |
| PX459 Puro | Addgene | Cat# 62988 |
| pLVX-mCherry-cGAS | This paper | N/A |
| Software and Algorithms | ||
| Graphpad Prism | GraphPad Software | https://www.graphpad.com/ |
| FlowJo | FlowJo Software | https://www.flowjo.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/index.html |