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Biochemical characterization of 8RK64. (A) IC50 determination of 8RK64 for UCHL1, UCHL3, and UCHL5. (B) Deconvoluted mass spectra of UCHL1 before (blue) and after (red) reaction with 8RK64. (C) Fluorescence labeling of the remaining DUB activity in HEK293T cells upon treatment with UCHL1 inhibitors 8RK64 and 6RK73.
