Table 3.
Validation of microarray results by MassARRAY QGE analysis (Sequenom), showing the fold change obtained from microarray analysis with TAC software and with Sequenom analysis. The fold change from Sequenom is obtained by dividing the molar concentration of each gene between Fracture and Control groups of women (six women each). p value for Sequenom analysis was obtained using a paired Student’s t test.
| Gene | Fold change from microarrays (TAC) | Fold change from Sequenom | p value |
|---|---|---|---|
| SULF2 | 2.53 | 2.09 | 0.010 |
| DSG3 | 1.83 | 1.99 | 0.013 |
| COL10A1 | 1.78 | 2.35 | 0.005 |
| RSPO3 | 1.69 | 2.03 | 0.024 |
| MEF2C | 1.55 | 1.28 | 0.507 |
| ADAM12 | − 1.68 | − 1.86 | 0.044 |
| GALNT5 | − 1.77 | − 1.13 | 0.682 |
| THSD4 | − 1.78 | − 1.77 | 0.005 |
| ITGA4 | − 2.07 | − 1.68 | 0.008 |
| FOSL1 | − 2.31 | − 1.49 | 0.418 |
| IL13RA2 | − 2.47 | − 2.84 | 0.002 |
| LEP | − 2.61 | − 2.84 | 0.00001 |