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. 2020 Oct 1;10:16240. doi: 10.1038/s41598-020-73448-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Difference in Fusobacterium spp. and F. nucleatum between colon, gastric mucosa and abundance in preneoplastic gastric mucosa. (A) Correlation between normalized Fusobacterium spp. and F. nucleatum in N-CRC and T-CRC specimens. (B) Correlation between normalized Fusobacterium spp. and F. nucleatum in N-GC and T-GC specimens. (C) Relative abundance of Fusobacterium spp. in N-CRC (n = 26) and N-GC (n = 78) (p < 0.0001). (D) Relative abundance of Fusobacterium spp. in T-CRC (n = 26) and T-GC (n = 79) (p < 0.0001). (E) Relative abundance of F. nucleatum in N-CRC (n = 25) and N-GC (n = 79) (p < 0.0001). (F) Relative abundance of F. nucleatum in T-CRC (n = 26) and T-GC (n = 80) (p < 0.0001). (G) Abundance of Fusobacterium spp. in N (n = 17), CNAG (n = 17), AG/IM (n = 6), N-GC (n = 78) and T-GC (n = 81) tissues in GC (p = 0.97). (H) Abundance of F. nucleatum in N (n = 18), CNAG (n = 17), AG/IM (n = 9), N-GC (n = 78) and T-GC (n = 80) in GC (p = 0.86). Relative abundance is presented as 2^ΔCT values normalized to PGT. Undetectable values were set to the lowest measurable normalized value. Mann–Whitney-test was used for statistical analysis of two groups and Kruskal–Wallis test for more than two groups.