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. 2020 Oct 1;25(39):2001650. doi: 10.2807/1560-7917.ES.2020.25.39.2001650

Table. Oligonucleotide primers used for amplification of SARS-CoV-2 nucleoprotein gene, Germany, July 2020.

Primer name Sequence
27870fwd GAAACTTGTCACGCCTAAACGAAC
28268fwd ACTAAAATGTCTGATAATGGACC
28943rev GCAGCAGCAAAGCAAGAGCAG
28923fwd CTGCTCTTGCTTTGCTGCTGC
29358rev GTTTTGTATGCGTCAATATGC
29338fwd GCATATTGACGCATACAAAAC
29588rev AGCGAAAACGTTTATATAGCCCATCTG
29880pArev TTTTTTTTTTGTCATTCTCCTAAGAAGCTATT

SARS-CoV-2: severe acute respiratory syndrome coronavirus 2.

All PCR reactions were performed with Q5-Polymerase (New England Biolabs GmbH, Frankfurt, Germany) including GC-Enhancer. Oligonucleotide primers were obtained from Integrated DNA Technologies, Coralville, United States. Primer names reflect the 5’ end of the respective oligonucleotide on the reference genome NC_045512.2 and are used throughout Figure 1.