Table. Oligonucleotide primers used for amplification of SARS-CoV-2 nucleoprotein gene, Germany, July 2020.
Primer name | Sequence |
---|---|
27870fwd | GAAACTTGTCACGCCTAAACGAAC |
28268fwd | ACTAAAATGTCTGATAATGGACC |
28943rev | GCAGCAGCAAAGCAAGAGCAG |
28923fwd | CTGCTCTTGCTTTGCTGCTGC |
29358rev | GTTTTGTATGCGTCAATATGC |
29338fwd | GCATATTGACGCATACAAAAC |
29588rev | AGCGAAAACGTTTATATAGCCCATCTG |
29880pArev | TTTTTTTTTTGTCATTCTCCTAAGAAGCTATT |
SARS-CoV-2: severe acute respiratory syndrome coronavirus 2.
All PCR reactions were performed with Q5-Polymerase (New England Biolabs GmbH, Frankfurt, Germany) including GC-Enhancer. Oligonucleotide primers were obtained from Integrated DNA Technologies, Coralville, United States. Primer names reflect the 5’ end of the respective oligonucleotide on the reference genome NC_045512.2 and are used throughout Figure 1.