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. Author manuscript; available in PMC: 2020 Oct 2.
Published in final edited form as: Cell Rep. 2020 Sep 1;32(9):108092. doi: 10.1016/j.celrep.2020.108092

Figure 6. Glia-Specific Ampk Deletion Causes Neuronal Death and Reduces Lifespan in Drosophila.

Figure 6.

(A–D) Semi-thin sections of brains (one hemisphere of the central brain and one optic lobe) of 50-day-old adult flies. Compared with controls (mCherry-dsRNA), AMPK knockdown in adult glial cells (using repo-Gal4, tub-Gal80ts) induces holes in the cortex region indicating neurodegeneration (arrows). Scale bars: 50 μm.

(E and F) Semithin sections of brains of 18-day-old adult flies. Adult glia-specific, but not adult neuron-specific, knockdown of the glycolytic gene Trehalase (Treh) induces neurodegeneration. AMPK knockdown albeit has a later onset and induces a similar phenotype. Scale bars: 50 μm.

(G-J) Semithin sections of brains of 50-day-old adult flies. Adult neuron-specific knockdown (using elav-Gal4, tub-Gal80ts) of AMPK does not alter brain structure. Scale bars: 50 μm.

(K and L) Kaplan-Meier survival curves of flies with AMPK knockdown in adult glial cells (K) or neurons (L), showing reduced lifespan of flies specifically with an adult glia-specific AMPK knockdown. n = 5 independent experiments; n > 300 flies/genotype. Statistics were performed using a Kaplan-Meier estimator in combination with a log rank test. Multiple comparison was performed using the Holm-Sidak method.