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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: J Am Chem Soc. 2020 Jan 3;142(2):874–883. doi: 10.1021/jacs.9b10066

Table 1:

Association constants used in the calculations.

Description Symbol no ficoll 4% ficoll Comment
SPOP polymerization ks (2.4 μM)−125 9.8 nM (6-16 nM) (range consistent with expts)
Monovalent DAXX eϵ (17 μM)−1 (10 μM)−1 Measured using SPOP ΔBACK
Divalent DAXX (vapor) e2ϵ+ln zv (1.0 μM)−1 (0.6 μM)−1 Measured using WT SPOP
Divalent DAXX (gel) e2ϵ+ln zg (0.5 μM)−1 (0.3 μM)−1 Assumes zg/zv ≃ 2