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. 2020 Sep 18;10:1780. doi: 10.3389/fonc.2020.01780

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Copyright © 2020 Singh, Devkar and Basu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of IL-6 following MyD88 inhibitor and TLR3 ligand treatment. (A) Fluorescent microscopy image of T47D cells, treated with TLR3 ligand (10 μg/ml) with or without MyD88 inhibitor (1 μM) following immunocytochemical staining with antibody against IL-6 and Alexa 488-tagged secondary antibody and counterstained with DAPI. Untreated indicates the cells are not treated with TLR3 ligand (magnification, 40X). (B) Bar graph showing the expression of IL-6 following observation through a microscope and analyses through the ImageJ software for all the experiment groups. (C,D) Expression of IL-6 in the cell culture supernatant as measured through ELISA. The results are presented as mean ± SD (p < 0.05 is treated as significant).