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. 2020 Sep 15;76(Pt 10):464–468. doi: 10.1107/S2053230X20011127

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Temperature (K) 288
Protein concentration (mg ml−1) 45
Buffer composition of protein solution 50 mM Tris–HCl pH 8, 50 mM NaCl
Reservoir solution 1.4 M sodium citrate tribasic dihydrate, 0.1 M HEPES-Na pH 7.5, 4% 1,3-butanediol
Volume and ratio of drop 4 µl, 2:2
Volume of reservoir (µl) 500