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. 2020 Sep 18;11:552490. doi: 10.3389/fgene.2020.552490

FIGURE 6.

FIGURE 6

Plant mtr-miR-5754 and miRgma-miR-4995 inhibitors reversed the effect of mtr-miR-5754 and miRgma-miR-4995 on metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and nuclear paraspeckle assembly transcript 1 (NEAT1) expression. (A) HCT116p53–/– cells were transfected with control microRNA (miRNA), mtr-miR-5754, or gma-miR-4995 and after 24 h with anti-miR5754 or anti-miR4995, respectively, which suppressed the plant miRNA levels in the cells. (B) Reverse transcription quantitative PCR (RT-qPCR) of MALAT1 and NEAT1 in HCT116p53–/– cells transfected with control miRNA, mtr-miR-5754, or gma-miR-4995 and after 24 h with anti-miR5754 or anti-miR4995, respectively (*p < 0.05; **p < 0.005; and ***p < 0.0005). (C) Cell Counting Kit-8 (CCK-8) assay of HCT116p53–/– cells transfected with control miRNA, mtr-miR-5754, or gma-miR-4995 and after 24 h with anti-miR5754 or anti-miR4995, respectively.