Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 19;12(1):1792130. doi: 10.1080/19420862.2020.1792130

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Maverick Therapeutics. Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Hemi-COBRA TDCC.

Mixtures of uncleaved and MMP9-cleaved hemi-COBRAs were tested in T cell dependent cellular cytotoxicity (TDCC) assays with primary human pan-T cells and HT-29 (A, B) or LoVo (C,D) tumor cells expressing luciferase. Tumor cell viability is indicated by relative luminescence units (RLU). EC₅₀ values for Pre-cleaved Pro268+ Pro60, cleaved Pro53+ Pro77, and Pro51 are indicated below. Accurate EC₅₀ values could not be calculated for other COBRAs. In both tumor cell lines, the fully active control Pro51 and mixtures of cleaved, complementary hemi-COBRAs (Pro268+ Pro60 and Pro53+ Pro77) were at least 500-fold more potent than non-EGFR binding Pro98 and mixtures of uncleaved or non-cleavable hemi-COBRAs.