Figure 5.

Hinge mutations decrease half-antibody formation. IgG4 mAbs with the indicated hinge mutation(s) were incubated with the components of the thioredoxin system. Samples were taken at the indicated timepoints and mAb fragments were quantified using capillary electrophoresis. Free heavy and light chain were not shown to aid in visualization, they are however, included in Fig S3. Data represent the mean ± SD of triplicate experiments.