Figure 6.

Hinge mutations decrease Fab-arm exchange. (a) Quantification of hybrid mAb formation for IgG4 mAbs with the indicated hinge mutation(s) after incubation with a second, unmodified IgG4. Hybrid mAb was quantified using capillary electrophoresis and quantification of each parent mAb is included in Fig. S4. Data represent the mean ± SD of triplicate experiments. (b) Example electropherogram from the capillary electrophoresis for the unmodified IgG4. (c) Example mass spectrometry data for the unmodified IgG4. Mass spectrometry for remaining samples are in Fig. S5 and S6.