Table 1.

Amino acid sequence comparison of the hinge region for IgG1, IgG2, and IgG4 antibodies using the EU index for antibody numbering. The positions of the mutations evaluated are indicated in red.

Position	215	216	217	218	219	220	221	222	223	224	225	226	227	228	229	230
IgG1 HC	V	E	P	K	S	C	D	K	T	H	T	C	P	P	C	P
IgG2 HC	V	E	R	K	C	C	-	-	-	V	E	C	P	P	C	P
IgG4 HC	V	E	S	K	Y	G	-	-	-	P	P	C	P	S	C	P