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. 2020 Aug 11;12(1):1803646. doi: 10.1080/19420862.2020.1803646

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Flow cytometry profiles for parental scFv yeast clones, epPCR, or VariantFind mutagenized libraries, and post-sort libraries. (a) EpPCR mutagenized samples. (b) VariantFind mutagenized samples. Each row from left to right represents the following samples: parental strain, unsorted mutagenized library, and one, two, or three times sorted libraries, all stained with the relevant soluble antigen. The y-axis reflects antigen binding, whereas the x-axis displays scFv expression through staining of the c-myc tag.