Table 3.
KD measurements for parental and VariantFind derived full-length antibodies and their corresponding scFv clones, measured using BLI.
| Full-length |
scFv |
||||
|---|---|---|---|---|---|
| Antibody | KD (nM) | Fold improved KD compared to parental clone | KD (nM) | Fold improved KD compared to parental clone | |
| VariantFind | tPD1.16 (parental) | 28.1 | - | 18.4 | - |
| rPD1.16.1 | 36.8 | 0.8 | 1.6 | 11.5 | |
| rPD1.16.2 | 12.3 | 2.3 | 1.7 | 10.8 | |
| tCTLA4.28 (parental) | 5.2 | - | 6.9 | - | |
| rCTLA4.28.1 | 1.2 | 4.2 | 0.5 | 13.8 | |
| tPDL1.3 (parental) | 1.8 | - | 0.2 | - | |
| rPDL1.3.1 | 1.0 | 0.8 | 0.1 | 3.2 | |
| tOX40.24 (parental) | 37.6 | - | 31.4 | - | |
| rOX40.24.1 | 8.9 | 4.2 | 2.2 | 14.3 | |
| rOX40.24.2 | 6.9 | 5.4 | 2.0 | 15.7 | |