Skip to main content
. 2020 Aug 2;12(1):1792673. doi: 10.1080/19420862.2020.1792673

Figure 3.

Figure 3.

(a) Binding of 10 nM and 60 nM FITC-labeled RTX-Fab to Daudi cells in the presence (red) and absence (blue) of 10 nM and 60 nM unlabeled RTX-Fab during dissociation, respectively. (b) Interaction Map analysis for 60 nM FITC-RTX-Fab binding to Daudi cells (c) Binding of 60 nM full-length RTX (green) and RTX-Fab2 (gray) to Daudi cells. Dissociation was recorded in plain cell culture medium for both. (d) The binding stability in the presence of ligand in solution was tested for the following association + dissociation combinations: FITC-RTX-IgG + unlabeled RTX-F(ab’)2 (gray), FITC-RTX-IgG + unlabeled RTX-IgG (green), FITC-RTX-F(ab’)2 + unlabeled RTX-IgG (pink) and for FITC-RTX-F(ab’)2 + unlabeled RTX-F(ab’)2(blue). (e) The percentage of molecules bound more stably as estimated by globally fitting a 1:2 model to the data displayed in (d).