Table 4.
Kinetics for RTX, OBI and OFA binding traces from Figures 1a, 4a and 5a as extracted with a 1:2 binding model.
| Interaction 1 | Interaction 2 | Contribution KD1 | |||||
|---|---|---|---|---|---|---|---|
| ka1 (1/(M*s)) | kd1 (1/s) | KD1 (M) | ka2 (1/(M*s)) | kd2 (1/s) | KD2 (M) | ||
| RTX | 1.56E+04 | 2.90E-06 | 1.86E-10 | 1.12E+04 | 1.82E-04 | 1.64E-08 | 76% |
| OBI | 9.25E+04 | 4.71E-06 | 5.09E-11 | 4.19E+04 | 3.64E-04 | 8.69E-09 | 56% |
| OFA# | 5.93E+04 | 3.42E-06 | 5.77E-11 | 1.13E+04 | 1.00E-06 | 8.85E-11 | 53% |
#Note that the interaction components defined for OFA by the 1:2 model do not correspond to monovalent and bivalent fraction.