Figure 3.

Lead ARG2 function-blocking clones appear to bind a common or overlapping epitope. Representative data showing that unlabeled C0020185 (Δ), C0020186 (▲) and C0020187 (□) scFv can compete away DyLight®650 labeled C0020187 scFv bound to human ARG2. Direct binding was measured using HTRF®. Labeled C0020187 scFv and biotinylated recombinant human ARG2 trimer were used at a fixed assay concentration of 100 nM and 12 nM, respectively. Assay data points represent the mean of duplicate wells ± standard deviation. Reciprocal competition experiments using DyLight®650-labeled C0020185 or C0020186 were also performed yielding similar results.