Figure 3.

CD24 is a stable marker for B cell depletion and indicates B-cell depletion more closely than CD19. WBLs were incubated for 4 h at 37°C in the presence of CD20 antibodies. The level of B-cell depletion was analyzed by flow cytometry. (a) Apparent loss of B cells mediated by IgA1, IgA2 and IgG1 CD20 antibodies. (b) Effect of increasing antibody concentration (IgA1 UMAB001) on CD19 levels. (c) Quantification of CD19 expression at several antibody concentrations, gated on CD24 + B cells (d) Quantification of CD24 expression on initially CD19 positive B cells. (e) Quantification of remaining B cells after whole blood leukocyte-mediated B cell depletion assay with CD20 antibodies gating on CD24 + B cells. One representative graph is shown for at least n = 3 independent experiments. Asterisks indicate statistically significant differences compared to the no Ab control.