Figure 2.
Equilibrium binding of GpL-tagged aCD40- and αCD95-scBaff fusion proteins to CD40 and CD95 and the Baff-interacting receptors BaffR, TACI and BCMA. (a,b) HEK293 transfectants transiently expressing the Baff-interacting receptors (a) or CD40 or CD95 (b) were used to determine total binding. Empty vector (EV) transfected cells were used to determine unspecific binding. Specific binding was calculated by subtraction of the unspecific binding values from the corresponding total binding values. Binding of GpL fusion proteins of TNC-Baff and TNC-APRIL was analyzed for comparison. Data of one representative experiment for each of the investigated interactions are shown. Averaged KD-values of 4 independent experiments are listed in Table 1.