Table 1.
Affinity of GpL-tagged TNC-Baff and CD40- and CD95-specific antibody-scBaff fusion proteins for cell-expressed receptors.
| Construct | KD values (nM) of independent experiments | Averaged KD (nM) |
|---|---|---|
| BaffR | ||
| TNC-Baff | 0.6, 0.6, 0.5, 0.5 | 0.5 ± 0.1 |
| αCD40N297A-scBaff | 0.5, 0.4, 0.2, 1.3 | 0.6 ± 0.5 |
| αCD40Fab2-scBaff | 0.4, 0.7, 0.4, 1.3 | 0.7 ± 0.4 |
| αCD95N297A-scBaff | 0.5, 0.5, 0.2, 1.2 | 0.6 ± 0.4 |
| αCD95Fab2-scBaff | 0.4, 0.6, 0.3, 0.8 | 0.5 ± 0.2 |
| BCMA | ||
| TNC-Baff | 2.2, 4.0, 1.1, 2.3 | 2.4 ± 1.2 |
| αCD40N297A-scBaff | 1.6, 2.5, 3.7, 3.6 | 2.8 ± 1.0 |
| αCD40Fab2-scBaff | 1.9, 2.9, 2.8, 2.1 | 2.4 ± 0.5 |
| αCD95N297A-scBaff | 1.4, 3.6, 4.3, 1.8 | 2.8 ± 1.4 |
| αCD95Fab2-scBaff | 1.5, 2.1, 2.6, 1.6 | 1.9 ± 0.5 |
| TACI | ||
| TNC-Baff | 2.9, 3.0, 2.2, 2.1 | 2.6 ± 0.5 |
| αCD40N297A-scBaff | 1.5, 2.0, 2.8, 2.9 | 2.3 ± 0.7 |
| αCD40Fab2-scBaff | 3.3, 1.6, 3.7, 2.0 | 2.6 ± 1.0 |
| αCD95N297A-scBaff | 1.5, 1.7, 3.0, 2.7 | 2.2 ± 0.7 |
| αCD95Fab2-scBaff | 2.0, 1.5, 1.8, 1.8 | 1.8 ± 0.2 |
| CD40 | ||
| αCD40-WT | 1.2, 1.3, 1.1, 1.0 | 1.1 ± 0.1 |
| αCD40N297A-scBaff | 1.4, 1.2, 1.7, 2.0 | 1.6 ± 0.3 |
| αCD40Fab2-scBaff | 1.8, 2.5, 4.1, 2.6 | 2.7 ± 1.0 |
| CD95 | ||
| αCD95-WT | 1.7, 1.9, 2.5, 3.8 | 2.5 ± 0.9 |
| αCD95N297A-scBaff | 1.7, 2.1, 3.0, 2.0 | 2.2 ± 0.5 |
| αCD95Fab2-scBaff | 4.6, 4.3, 4.9, 4.4 | 4.5 ± 0.3 |