| Strain |
|
|
|
E. coli
|
|
|
| Top 10 |
Cloning host |
Invitrogen |
| CA434 |
Conjugation host |
[25] |
|
C. difficile
|
|
|
| 630Δerm
|
Erythromycin sensitive clone of CD630 |
[26] |
| 630ΔermΔpyrE
|
pyrE mutant to facilitate AE mutagenesis |
[20] |
| 630ΔermΔpyrEΔtcdA
|
Initial tcdA-deletion mutant |
This study |
| 630ΔermΔtcdA
|
pyrE-restored tcdA-deletion mutant |
This study |
| 630ΔermΔpyrEΔtcdA.TcdB::GTD D270N |
Initial GTD-defective mutant |
This study |
| 630ΔermΔtcdA.TcdB::GTD D270N |
pyrE-restored GTD-defective mutant |
This study |
| Plasmid |
|
|
| pMTL-YN3 |
Knockout vector for 630Δerm
|
[20] |
| pMTL-YN3-tcdA KOC |
As above with knockout cassette for tcdA
|
This study |
| pMTL-YN3-TcdB::GTD D270N SC |
As above with substitution cassette for TcdB::GTD D270N |
This study |
| pMTL-YN1 |
pyrE-restoration vector for 630Δerm
|
[20] |
