FIG 3.
High-performance anion-exchange chromatography (HPAEC) profiles of supernatants collected during the growth of B. ovatus ATCC 8483T, S. variabile DSM 15176T, and H. hathewayi DSM 13479T in pure culture during anaerobic incubation at 37°C. Peaks corresponding to DP2 (probably laminaribiose), DP3 (3-O-β-cellobiosyl-d-glucose), and DP4 (3-O-β-cellotriosyl-d-glucose) oligosaccharides are indicated by reference to standards. Sampling times are indicated, and chromatographic profiles of samples that were analyzed with whole-transcriptomics analysis (RNA-seq) are colored red/orange. (A) B. ovatus ATCC 8483T supernatants from barley β-glucan medium. (B) H. hathewayi DSM 13479T supernatants from oligosaccharide medium. (C) S. variabile DSM 15176T supernatants from oligosaccharide medium.