TABLE 3.

Differential transcription of genes involved in degradation of barley β-glucan in B. ovatus ATCC 8483^T in pure culture and coculture^a

Gene name	Product (EC no.)	Pure culture (batch)b			Coculture (batch)b				Coculture (continuous)b
		Log2FC BO16 vs BO20	Log2FC BO16 vs BO26	FDR	Log2FC BOHH24 vs BO24	Log2FC BOSV24 vs BO24	Log2FCBOSVHH24 vs BO24	FDR	Log2FC BOSVHH vs BO	FDR
Bovatus_RS15290	Glycoside hydrolase family 16 (GH16; EC 3.2.1.-)	3.786	7.074	1.55e-04	6.695	–c	5.395	1.26e-03	–0.037	0.955
Bovatus_RS15295	SusC/RagA family TonB-linked outer membrane protein	2.819	6.775	3.69e-04	6.413	1.180	5.398	9.49e-04	–0.428	0.358
Bovatus_RS15300	SusC/RagB family nutrient-binding outer membrane lipoprotein	2.734	6.676	2.86e-04	6.113	1.043	5.364	1.52e-03	–0.696	0.083
Bovatus_RS15305	Hypothetical protein	2.507	7.055	1.23e-04	7.252	1.912	6.557	2.73e-04	–0.769	0.053
Bovatus_RS15310	β-Glucosidase (EC 3.2.1.-)	2.542	6.996	5.05e-04	6.862	1.345	6.159	1.12e-03	–0.686	0.080

^aGene name, product, Log2FC (fold change) of each comparison, and FDR (false discovery rate) are reported. Genes showing an FDR of <0.05 and absolute value of Log2FC of >1 were considered statistically differentially transcribed.

^bBO, B. ovatus; BOHH, coculture of B. ovatus and H. hathewayi; BOSV, coculture of B. ovatus and S. variabile; BOSVHH, coculture of B. ovatus, S. variabile, and H. hathewayi. Comparisons in pure culture are between transcription at 16 h and 20 or 26 h of incubation. Comparisons in coculture are between pure culture and coculture, both at 24 h of incubation.

^c–, absolute value Log2FC < 1.