Table 1. Effects of mutations on neurosteroid modulation of [3H]muscimol binding.
EC50, Hill slope and maximal effect values [Emax (% of control): 100% means no effect] for the concentration-response curves in Figure 6B–E. Statistical differences are analyzed using one-way ANOVA with Bonferroni's multiple comparisons test (*p<0.05 vs. WT; **p<0.01 vs. WT). Data are presented as mean ± SEM.
| 3α5αP | EC50 (μM) | Hill slope | Emax (% of control) | N |
|---|---|---|---|---|
| WTα1β3 | 0.24 ± 0.04 | 1.10 ± 0.14 | 374.1 ± 11.1 | 6 |
| α1(Q242L)β3 | **2.66 ± 0.51 | 1.16 ± 0.37 | **159.8 ± 10.9 | 6 |
| α1(N408A/Y411F)β3 | **2.30 ± 0.48 | 0.87 ± 0.44 | **146.0 ± 9.3 | 6 |
| α1β3(Y284F) | 0.19 ± 0.04 | 0.87 ± 0.16 | 342.3 ± 13.9 | 6 |
| α1(Q242L/N408A/Y411F)β3 | - | - | **105.9 ± 7.3 | 6 |
| 3β5αP | ||||
| WTα1β3 | 0.25 ± 0.08 | 0.84 ± 0.23 | 195.1 ± 6.7 | 3 |
| α1(Q242L)β3 | 0.27 ± 0.09 | 0.77 ± 0.21 | 204.3 ± 4.5 | 3 |
| α1(N408A/Y411F)β3 | 0.61 ± 0.26 | 2.25 ± 0.92 | **124.3 ± 2.6 | 3 |
| α1β3(Y284F) | - | - | **148.6 ± 4.9 | 3 |
| KK148 | ||||
| WTα1β3 | 2.40 ± 0.36 | 1.36 ± 0.16 | 431.0 ± 19.5 | 6 |
| α1(Q242L)β3 | 2.20 ± 0.31 | 1.24 ± 0.12 | 434.5 ± 5.6 | 6 |
| α1(N408A/Y411F)β3 | 1.63 ± 0.53 | 0.73 ± 0.23 | **161.7 ± 3.5 | 6 |
| α1(V227W)β3 | 1.73 ± 0.68 | 0.76 ± 0.31 | **209.2 ± 7.4 | 6 |
| α1β3(Y284F) | 1.79 ± 0.44 | 1.35 ± 0.13 | **357.2 ± 8.1 | 6 |
| Etomidate | ||||
| WTα1β3 | 7.24 ± 1.18 | 1.07 ± 0.17 | 331.1 ± 9.9 | 6 |
| α1(Q242L)β3 | 7.50 ± 0.95 | 1.35 ± 0.20 | **277.8 ± 10.9 | 6 |
| α1(N408A/Y411F)β3 | 9.14 ± 2.20 | 1.07 ± 0.26 | **268.2 ± 5.9 | 6 |
| α1β3(Y284F) | 7.71 ± 1.10 | 0.90 ± 0.11 | 303.5 ± 5.8 | 6 |
| α1β3(M286W) | *22.5 ± 6.17 | 0.50 ± 0.16 | **128.6 ± 7.8 | 6 |