Fig. 1. Assaying D-serine’s efficacy in preventing temporal lobe epilepsy.

a Schematic and details of the route and mode of D-serine administration. Note changes in stereotaxic coordinates required to accurately target the right MEA with a cannula (confirmed through injection of India ink, inset) as a function of age and body weight of the rat (gray area indicates range of animals used). The non-cannulated cohort of animals includes those utilized in past studies of TLE in the laboratory^32,33. The MEA is also referred to as area entorhinalis pars medialis in the literature³⁴. b Tree diagram showing behaviorally observed outcomes (status epilepticus, frank seizures, epilepsy or death) of pilocarpine treatment (insult) under conditions identified in a. The boxed numbers indicate percent of total animals (red) or percent of animals with status epilepticus (status, blue), under various regimens (changes are flagged to encourage comparison). All video recordings of behavior are available upon request.