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. 2020 Oct 2;10:16377. doi: 10.1038/s41598-020-72454-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of C5a on neutrophil function: killing and phagocytosis. Purified neutrophils were treated with or without C5a (1–100 ng/ml) for 16 h prior to functional assay. (A) Killing expressed as viable counts of MRSA (cfu/ml). (B) Phagocytosis expressed as the percentage of neutrophils containing MRSA. Data are expressed as the mean ± SEM of 4 separate donors. *Represents a significant difference between viable counts of MRSA at t = 0 versus t = 2 with neutrophils present. **Represents a significant difference between viable counts of MRSA at t = 2 with neutrophils present versus t = 2 with neutrophils present treated with C5a at 100 ng/ml. ***Represents a significant difference in phagocytosis between neutrophils treated and not treated with C5a.