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. 2020 Oct 2;11:4974. doi: 10.1038/s41467-020-18477-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Affinity tag-containing dummy chains drive bsAb assembly and enable one-step purification. b Combination of educts of different formats generates bsAb format diversity. Green circles indicate the location of paratopes in output formats. c Combining binders in different input formats multiplies the binder–format design space. Each product within the matrix is a unique bsAb with a specific binder combination in a specific format.