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. 2020 Sep 24;2020:2452848. doi: 10.1155/2020/2452848

Figure 3.

Figure 3

Effect of WFA on PDGF-BB-induced liver fibrosis and the expression of SIRT3 in activated JS1 cells. JS1 cells were treated with DMSO (0.02%, w/v), PDGF-BB (20 ng/ml), and the indicated concentrations of WFA for 24 h. (a–c) Quantification of α-SMA, fibronectin, and α1(I) procollagen expression in JS1 cells were carried out by real-time PCR and Western blot. (d) Quantification of SIR2 family (SIRT1-SIRT7) mRNA expression was assessed by real-time PCR. (e) The effect of WFA on the enzyme activity of SIRT3 deacetylase in activated JS1 cells. (f) Quantification of SIRT3 protein expression was assessed by Western blot in activated JS1 cells. For the statistics of each panel in this figure, data are expressed as mean ± SD (n = 3). ∗p < 0.05 and ∗∗p < 0.01.