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. 2020 Sep 24;2020:2452848. doi: 10.1155/2020/2452848

Figure 4.

Figure 4

Effect of SIRT3 depletion on the antifibrogenic effect of WFA in activated JS1 cells. (a) After SIRT3 siRNA or NC siRNA was transfected into JS1 cells for 24 h, SIRT3 protein expression was measured with Western blots. (b) After SIRT3 siRNA or NC siRNA was transfected into JS1 cells for 24 h, cells were treated with DMSO (0.02%, w/v), PDGF-BB (20 ng/ml), and the indicated concentrations of WFA for 24 h. SIRT3 protein expression was measured by Western blots. (c–e) Quantification of α-SMA, fibronectin, and α1(I) procollagen expression in SIRT3-siRNA-treated or untreated JS1 cells (with NC siRNA transfection). For the statistics of each panel in this figure, data are expressed as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.