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. 2020 Oct 2;11:4964. doi: 10.1038/s41467-020-18802-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a During CCl₄-induced acute liver injury (ALI), thrombocytosis was detected as an increased peripheral blood (PB) platelet counts in the ALI vs. control (CON) mice (n = 12 mice, two-way repeated-measures ANOVA with Bonferroni’s multiple comparison tests, p = 0.0045 (Con as control)). Non-treated mice (WT) were set as baseline control. b, c Megakaryocytopoiesis in bone marrow (BM) after CCl₄ injection is shown. On day 8 after injection, representative femur sections stained with CD41 and hematoxylin showed increased levels of CD41-positive cells (Scale bars: 100 μm) (b), and a histogram indicated that BM whole megakaryocytic cells (MK, CD41 positive), including mature megakaryocytes (m-MK, CD41, and CD42 double-positive) and megakaryocyte-erythroid progenitors (MEP, CD41, and CD71 double positive), were increased in the ALI mice c. Representative FCM plots are shown in Supplementary Fig. 1g. d The percentage of cells of each ploidy in BM is shown. e, f Liver TPO synthesis was suppressed under ALI, as demonstrated by liver cell qPCR (e) and plasma TPO ELISA analyses (two-way repeated measures ANOVA with Bonferroni’s multiple comparison tests, p = 0.0085) (f). g PB-PLTs incorporated increased levels of CD62p-positive activated PLT in the ALI mice on day 2 after injection. h–j Platelet-derived microparticles (PMPs) increased in the ALI mice (n = 12 mice). Compared with those of the control group, the circulating CD41⁺ microparticles (MPs) increased (two-way repeated-measures ANOVA with Bonferroni’s multiple comparison tests) (h), and the proportion of CD41⁺/CD62p⁺ PMPs among the CD41⁺ MPs in PB increased as well (two-way repeated-measures ANOVA with Bonferroni’s multiple comparison tests, p = 0.0109) (i). The absolute count of PMPs on day 2 after CCl₄ injection was higher than that in the control group when platelets started to increase mice) (j). Each time point in each animal cohort represents a mean ± S.E.M. of nine mice and two-tailed unpaired t-tests were used unless otherwise specified. Source data are provided as a Source Data file *P < 0.05, **P < 0.01, ***P < 0.001.