Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 2;11:4964. doi: 10.1038/s41467-020-18802-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Venn diagrams were generated for the highly expressed miRNAs in all five subjects. b Heatmaps of the highly expressed miRNA contents of the PMPs from five different donors are shown. c Relative expression of the 25 most abundant miRNAs in the PMPs was analyzed by qPCR. miRNA expression is shown relative to miR-4454 (top panel) and the cell number (bottom panel) (n = 3 donors) d The cells were transfected with 7 miRNA mimics sorted from the expression profile and cultured in medium containing 1 nM PMA to induce megakaryocytic differentiation. The impact of miRNA overexpression on these megakaryocytic cell lines was determined by FCM. Altered expression of the miRNAs influenced the expression of the MK integrins CD41 and CD61 (n = 3 independent experiments, one-way ANOVA with Dunnett’s test, NC as control). e Relative expression of the 6 selected miRNAs in mouse platelets (left) and PMPs (right) 2 days after CCl₄ injection was analyzed by qPCR. miRNA expression is shown relative to the platelet or PMP number (n = 9 mice). f qPCR analysis of miR-1915-3p expression in BM-nucleated cells of the ALI mouse (n = 9 mice). All data are shown as the mean ± S.D from two-tailed unpaired-samples t-tests unless otherwise specified. P-values. Source data are provided as a Source Data file *P < 0.05, **P < 0.01, ***P < 0.001.