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. 2020 Oct 2;10:16395. doi: 10.1038/s41598-020-73390-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antigenicity of gTSSA-I is due to TSSA-I sequence. (a) Absorbance values with Colombian (Medellín) sera recognising gTSSA-I (blue bars) and gTSSA-II/V/VI (orange bars). (b) Overall absorbance values for Colombian (Medellín) sera against gTSSA-I (blue) and gTSSA-II/V/VI (orange); gTSSA-I is recognised, whereas gTSSA-II/V/VI is not (P < 0.0001). (c) ELISA plate illustrating the recognition of gTSSA-I but not of gTSSA-II/V/VI; all samples were seropositive with lysate, and coating buffer controls were negative. Sample numbers correspond with (a). Positive control: serum from a Bolivian patient previously shown to be reactive with synthetic peptide TSSApep-II/V/VI and also seropositive with gTSSA-I, indicating co-infection (see text).