Figure 5.

miR-424-5p induced proliferation and migration promotion are rescued by overexpression of SIRT4. (A) The expression level of SIRT4 protein in EC-9706 overexpressing miR-424-5p was detected by western blot after the cells were transfected with SIRT4 or empty vector. (B) CCK-8 and (C) Colony-forming assays were performed to examine the effects of SIRT4 on proliferative ability of miR-424-5p overexpressing EC-9706 cells. (D) Wound healing assay and (E) Transwell assay were conducted to determine the roles of SIRT4 on the cell migration of EC9706-miR-424-5p compared with EC9706-NC. Data are presented as mean ± SD of three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001.