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Primer sequences for RT and qRT-PCR.
| Name | Sequence (5'-3') |
|---|---|
| RT primers | |
| U6 | CGAGCACAGAATCGCTTCACGAATTTGCGTGTCAT |
| miR-135-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACATAG |
| qRT-PCR primers | |
| U6 | F: CGAGCACAGAATCGCTTCA; |
| R: CTCGCTTCGGCAGCACATAT | |
| miR-135-5p | F: CAGTGCAGGGTCCGAGGTAT; |
| R: CGTCGTATGGCTTTTTATTCC | |
| SMAD3 | F: CCATCTCCTACTACGAGCTGAA; |
| R: CACTGCTGCATTCCTGTTGAC | |
| β-actin | F: CATGTACGTTGCTATCCAGGC; |
| R: CTCCTTAATGTCACGCACGAT |
F: forward; R: reverse.
